circRNA basic information
circBase ID: hsa_circ_0005588
Name: hsa_circ_WBSCR22
Synonym: circWBSCR22
Host Gene: WBSCR22
Genomic location(hg19): chr7:73100965-73101425:+
Genomic location(hg38): chr7:73686635-73687095:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissue; adjacent normal tissue; tumour tissue; liver tissue; subcutaneous tumours
Cell lines:

HCT116; SW480; HEK293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: UPF1, CHD4
Associated microRNA: -
Biological function: Promotes CRC cell proliferation, migration, invasion, EMT, tumour growth and metastasis in vitro and in vivo.
Molecular mechanism: circWBSCR22 directly binds the RBP UPF1, attenuates UPF1-mediated ubiquitination degradation of CHD4, stabilizes CHD4 protein, and thereby promotes EMT and CRC metastasis.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ubiquitination (inhibits)
Detected method:
Q
H
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; ISH (In Situ Hybridization); IHC (Immunohistochemistry); IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; Transfection; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; Western Blot; Bioinformatics Analysis
Clinical significance:

Potential biomarker and therapeutic target for CRC diagnosis, prognosis, clinical treatment guidance, and metastatic CRC treatment.
Description:

circWBSCR22 (hsa_circ_0005588) is up-regulated in colorectal cancer tissues and functions as an oncogenic circRNA. It promotes CRC proliferation, migration, invasion, EMT, tumour growth, and metastasis by binding UPF1 and reducing UPF1-mediated ubiquitination degradation of CHD4, thereby stabilizing CHD4 protein. The study proposes circWBSCR22 as a potential diagnostic/prognostic biomarker and therapeutic target for CRC.
Confidence score:

0.8817
Other information
Title:

Circular RNA circWBSCR22 facilitates colorectal cancer metastasis by enhancing CHD4's protein stability.

Journal: International journal of biological macromolecules
Published: 2024
PubMed ID: 39486700
Study type:

combined biological and clinical study
Data availability: GSE138589; GSE126094; Supplementary Materials; https://doi.org/10.1016/j.ijbiomac.2024.137135
Code availability: -