circRNA basic information
circBase ID: hsa_circ_0005100
Name: hsa_circ_FMN2
Synonym: circRNA formin 2 / Circ_0005100
Host Gene: FMN2
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: extracellular vesicle
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008315
MONDO name: prostate cancer
Disease details: prostate cancer / PCa
Disease DO ID:
 10283
Disease MeSH ID:
 D011471
Disease NCIt ID:
 C7378
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

PC tissues; adjacent non-cancerous tissues; xenograft tumors; lung tissues; liver tissues
Cell lines:

PC-3; DU145; C4-2; 22RV1; RWPE-1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: HuR, KLF2, RNF128
Associated microRNA: -
Biological function: Promotes PC cell proliferation, invasion, migration, tumorigenesis, and lung/liver metastasis.
Molecular mechanism: Tumor-derived EVs shuttle circFMN2 into PC cells; circFMN2 binds HuR, decreases HuR-KLF2 interaction, destabilizes KLF2 mRNA, represses KLF2 expression, reduces KLF2 binding to the RNF128 promoter, and represses RNF128 transcription.
Biological pathway or process:

proliferation (promotes); invasion (promotes); migration (promotes); metastasis (promotes); mRNA stability (inhibits); other pathway/process (other)
Detected method:
Q
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; IF (Immunofluorescence); Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis
Clinical significance:

circFMN2 expression was associated with tumor stage, lymph node metastasis, and Gleason score; PC-EVs-derived circFMN2 may serve as a potential diagnostic and therapeutic molecular target.
Description:

circFMN2 is upregulated in prostatic cancer tissues and cell lines and is shuttled between PC cells by tumor-derived extracellular vesicles. It promotes proliferation, invasion, migration, tumor growth, and lung/liver metastasis by binding HuR, reducing HuR-mediated KLF2 mRNA stability, and repressing the KLF2/RNF128 transcriptional axis. Clinically, higher circFMN2 expression correlates with tumor stage, lymph-node metastasis, and Gleason score.
Confidence score:

0.8644
Other information
Title:

Mechanism of tumor-derived extracellular vesicles in prostatic cancer progression through the circFMN2/KLF2/RNF128 axis.

Journal: Apoptosis : an international journal on programmed cell death
Published: 2023
PubMed ID: 37452271
Study type:

combined biological and clinical study
Data availability: available from the corresponding author on reasonable request; Supplementary information available at https:// doi. org/ 10. 1007/ s10495-023-01872-y
Code availability: -