| Expression pattern: |
DN |
| Associated gene: |
DLG2 mRNA, DLG2, YAP, Ago2 |
| Associated microRNA: |
miR-942-5p |
| Biological function: |
Suppresses CRC tumor progression in vivo; inhibits CRC cell survival, proliferation, colony formation, migration, invasion and lung metastasis; promotes apoptosis and S phase cell cycle arrest. |
| Molecular mechanism: |
circ0106714 acts as a miR-942-5p sponge, releases DLG2, and promotes YAP phosphorylation through Hippo-YAP signaling to suppress CRC progression. |
| Biological pathway or process: |
Hippo/YAP (promotes); apoptosis (promotes); cell cycle (other); proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Microarray; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis; Nuclear-Cytoplasmic Fractionation |
| Clinical significance: |
circ0106714 could serve as a potential molecular target for the prognosis and therapy of CRC. |
| Description: |
circ0106714 is a human TADA2A-derived circRNA that is significantly downregulated in colorectal cancer tissues and cell lines. It functions as a tumor suppressor by sponging miR-942-5p, releasing DLG2, and promoting YAP phosphorylation through Hippo-YAP signaling, thereby inhibiting proliferation, migration, invasion, metastasis and promoting apoptosis/cell-cycle arrest. |
| Confidence score: |
0.8256 |