| Expression pattern: |
UP |
| Associated gene: |
MAP4K4, AGO2 |
| Associated microRNA: |
miR-145-5p |
| Biological function: |
Promotes HCC proliferation, migration, tumorigenesis, and tumor progression; circCPSF6 knockdown inhibits HCC cell proliferation and migration and reduces xenograft tumor growth. |
| Molecular mechanism: |
circCPSF6 acts as a competing endogenous RNA that sponges miR-145-5p and upregulates the miR-145-5p target gene MAP4K4. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; Microarray; Northern Blot; FISH / smFISH; IHC (Immunohistochemistry); RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; In Vivo Animal Model; H&E Staining; Western Blot; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
circCPSF6 may serve as a potential HCC biomarker and treatment target; its expression was associated with clinicopathological characteristics in the Lanzhou II cohort. |
| Description: |
circCPSF6 is up-regulated in human HCC tissues and cell lines and functions as a pro-tumorigenic circRNA. It promotes HCC proliferation, migration, and xenograft tumor growth by sponging miR-145-5p and thereby upregulating MAP4K4. The circCPSF6-miR-145-5p-MAP4K4 axis is proposed as a potential biomarker and therapeutic target in HCC. |
| Confidence score: |
0.8658 |