| Expression pattern: |
UP |
| Associated gene: |
SIRT1, E-cadherin, Vimentin, p65, phosphorylated p65 |
| Associated microRNA: |
miR-204, miR-204-5p |
| Biological function: |
Promotes GC cell proliferation, migration, invasion, metastasis, EMT, cell cycle changes and apoptosis; high expression is associated with tumor stage and poor prognosis. |
| Molecular mechanism: |
circNOP10 acts as a competing endogenous RNA sponge for miR-204, regulates SIRT1 expression, mediates EMT, and promotes GC progression through the NF-kappabeta pathway. |
| Biological pathway or process: |
NF-kappaB (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); apoptosis (promotes); cell cycle (other); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; FISH / smFISH; RNA Pull-Down; Luciferase Reporter Assay; Transfection; Western Blot; CCK8; Transwell Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Increased circNOP10 in GC tissue was involved in tumor stage and prognosis; high circNOP10 expression was related to decreased overall survival and was an independent prognostic factor; it may serve as a prognostic biomarker and potential therapeutic target. |
| Description: |
circNOP10/hsa_circ_0034351 is up-regulated in human gastric cancer tissues and peripheral blood cells, and high expression is associated with tumor stage, metastasis and poor overall survival. Mechanistically, circNOP10 acts as a miR-204 sponge to regulate SIRT1 and activate NF-kappabeta signaling, promoting malignant phenotypes including proliferation, migration, invasion and EMT. |
| Confidence score: |
0.8423 |