circRNA basic information
circBase ID: hsa_circ_0004771
Name: hsa_circ_NRIP1
Synonym: -
Host Gene: NRIP1
Genomic location(hg19): chr21:16386664-16415895:-
Genomic location(hg38): chr21:15014343-15043574:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0003060
MONDO name: biliary tract cancer
Disease details: biliary tract cancer / BTC
Disease DO ID:
 4607
Disease MeSH ID:
 -
Disease NCIt ID:
 -
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BTC tumor tissues; corresponding normal tissues; adjacent normal bile duct tissues
Cell lines:

HIBEC; CCLP-1; QBC939; RBE; HCCC-9810
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: AKT2, PI3K/AKT/mTOR signaling pathway
Associated microRNA: miR-515-5p
Biological function: Promotes BTC tumor cell proliferation, invasion, migration, stemness maintenance, epithelial-mesenchymal transition, tumor growth, and metastasis.
Molecular mechanism: circ_NRIP1 functions as a competing endogenous RNA that sponges miR-515-5p, upregulates AKT2 expression, and activates the PI3K/AKT/mTOR signaling pathway.
Biological pathway or process:

PI3K/AKT/mTOR (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); stemness (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; Cohort Study; Survival Analysis; ROC Analysis; Bioinformatics Analysis
Clinical significance:

High circ_NRIP1 expression is associated with advanced TNM stage, lymph node invasion, poorer overall survival, and diagnostic potential in BTC.
Description:

circ_NRIP1 is overexpressed in BTC tissues and cells and is associated with advanced disease features and poor overall survival. Functionally, circ_NRIP1 promotes proliferation, invasion, migration, stemness, EMT, xenograft growth, and metastasis. Mechanistically, it sponges miR-515-5p to upregulate AKT2 and activate the PI3K/AKT/mTOR pathway.
Confidence score:

0.8658
Other information
Title:

Circular RNA nuclear receptor interacting protein 1 promoted biliary tract cancer epithelial-mesenchymal transition and stemness by regulating the miR-515-5p/AKT2 axis and PI3K/AKT/mTOR signaling pathway.

Journal: Environmental toxicology
Published: 2023
PubMed ID: 37466171
Study type:

combined biological and clinical study
Data availability: -
Code availability: -