| Expression pattern: |
UP |
| Associated gene: |
CNOT7, CCR4-NOT transcription complex and subunit 7, beta-catenin, cyclin D1, c-myc, Wnt signaling |
| Associated microRNA: |
miR-578 |
| Biological function: |
Promotes osteosarcoma cell proliferation, migration, and invasion; inhibits apoptosis; contributes to osteosarcoma progression. |
| Molecular mechanism: |
hsa_circ_001350 functions as a miR-578 sponge to increase CNOT7 protein levels and activate Wnt signaling, including beta-catenin, cyclin D1, and c-myc expression. |
| Biological pathway or process: |
Wnt/beta-catenin (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
hsa_circ_001350, miR-578, and CNOT7 may be potential targets for the treatment of OS. |
| Description: |
hsa_circ_001350 is up-regulated in osteosarcoma samples and cell lines and acts as an oncogenic circRNA. It promotes OS cell proliferation, migration, and invasion while suppressing apoptosis by sponging miR-578, thereby increasing CNOT7 protein levels and activating Wnt/beta-catenin-related signaling. The study suggests that hsa_circ_001350 and its miR-578/CNOT7/Wnt axis may be therapeutic targets in osteosarcoma. |
| Confidence score: |
0.7204 |