circRNA basic information
circBase ID: -
Name: mmu_circ_FOXP1
Synonym: circ057 / circFOXP1
Host Gene: FOXP1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0002771
MONDO name: pulmonary fibrosis
Disease details: pulmonary fibrosis
Disease DO ID:
 3770
Disease MeSH ID:
 D011658
Disease NCIt ID:
 C26869
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Mouse
Species details: Mus musculus
Tissue specimen:

lung tissue
Cell lines:

MRC-5
In vivo animal model:

other disease animal model
circRNA-disease information
Expression pattern:
UP
Associated gene: HuR, EZH2, STAT1, FOXK1, ULK1, ATG5, DRAM2, GABARAP
Associated microRNA: -
Biological function: circFOXP1 functions as a profibrotic factor; circFOXP1 knockdown blocks TGF-beta1-treated fibroblast proliferation and migration and reduces fibrotic protein expression, whereas circFOXP1 overexpression promotes proliferation, migration, fibrotic protein expression, and reverses the anti-fibrotic effect of hucMSCs treatment.
Molecular mechanism: circFOXP1 binds HuR and supports HuR expression, nucleocytoplasmic shuttling, and stability; hucMSCs treatment downregulates circFOXP1, blocks HuR nuclear-to-cytoplasmic translocation, promotes HuR degradation, decreases EZH2/STAT1/FOXK1, and enhances autophagy to ameliorate pulmonary fibrosis.
Biological pathway or process:

fibrosis (promotes); proliferation (promotes); migration (promotes); autophagy (inhibits); mRNA stability (promotes); other pathway/process (other)
Detected method:
Q
Validation methods:

RT-qPCR; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Transfection; CCK8; Wound Healing Assay; IF (Immunofluorescence); Western Blot; In Vivo Animal Model; H&E Staining
Clinical significance:

-
Description:

circFOXP1 is up-regulated in pulmonary fibrosis models and acts as a profibrotic circRNA. hucMSCs treatment downregulates circFOXP1, which reduces HuR stability and nucleocytoplasmic shuttling, decreases EZH2/STAT1/FOXK1, enhances autophagy, and thereby attenuates pulmonary fibrosis.
Confidence score:

0.5706
Other information
Title:

hucMSCs Treatment Ameliorated Pulmonary Fibrosis via Downregulating the circFOXP1-HuR-EZH2/STAT1/FOXK1 Autophagic Axis.

Journal: Stem cells (Dayton, Ohio)
Published: 2023
PubMed ID: 37419489
Study type:

biological research
Data availability: All data generated and/or analyzed during this study are included in this published article.
Code availability: -