| Expression pattern: |
DN |
| Associated gene: |
predicted target genes |
| Associated microRNA: |
- |
| Biological function: |
circRNA_3238 is a stable circular transcript mainly localized in the cytoplasm and may indirectly regulate the ALV-J infection process through predicted target genes. |
| Molecular mechanism: |
Predicted ceRNA mechanism involving circRNA_3238-miRNA-mRNA network and enrichment of predicted target genes in MAPK signaling pathway, Notch signaling pathway, Toll-like receptor signaling pathway and other pathways. |
| Biological pathway or process: |
ceRNA regulation (other); MAPK (other); Notch (other); immune regulation (other); other pathway/process (other) |
| Detected method: |
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Transfection; Bioinformatics Analysis; RNA-seq |
| Clinical significance: |
- |
| Description: |
circRNA_3238 is a chicken Foxp1-derived exonic circular RNA that is significantly down-regulated after ALV-J infection according to RNA-seq screening. The study experimentally confirmed its circular structure, stability and cytoplasmic localization, and bioinformatics analysis suggested that it may regulate ALV-J infection through a circRNA-miRNA-mRNA ceRNA network involving MAPK, Notch and immune-related pathways. |
| Confidence score: |
0.6149 |