| Expression pattern: |
UP |
| Associated gene: |
PDLIM5, Smad2, COL1A2, KLF4, TGF-beta, Smad3 |
| Associated microRNA: |
- |
| Biological function: |
circZBTB46 inhibits TGF-beta-induced Smad signaling and downregulates COL1A2/type I collagen synthesis in human vascular smooth muscle cells, contributing to vascular homeostasis and aneurysm development. |
| Molecular mechanism: |
circZBTB46 acts through a protein-scaffold mechanism by binding PDLIM5 and mediating the PDLIM5-Smad2 interaction, thereby inhibiting TGF-beta/Smad signaling and reducing COL1A2 expression. |
| Biological pathway or process: |
TGF-beta/SMAD (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
H
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; Transfection; Western Blot; IF (Immunofluorescence); H&E Staining |
| Clinical significance: |
circZBTB46 expression is increased in human AAA tissues and may represent a potential target for TGF-beta-induced ECM remodeling. |
| Description: |
circZBTB46 is a human ZBTB46-derived circRNA that is increased in abdominal aortic aneurysm tissues. In VSMCs, it binds PDLIM5 and promotes the PDLIM5-Smad2 interaction, inhibiting TGF-beta/Smad signaling and suppressing COL1A2/type I collagen expression, suggesting a role in vascular ECM remodeling and aneurysm development. |
| Confidence score: |
0.8115 |