| Expression pattern: |
UP |
| Associated gene: |
SLC7A11 |
| Associated microRNA: |
miR-431 |
| Biological function: |
Promotes CRC cell proliferation and suppresses ferroptosis; circSTIL knockdown retards proliferation and induces ferroptosis of CRC cells. |
| Molecular mechanism: |
circSTIL functions as a ceRNA by sponging miR-431 to regulate SLC7A11 expression, thereby mediating CRC progression and ferroptosis-related phenotypes. |
| Biological pathway or process: |
proliferation (promotes); ferroptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circSTIL expression is correlated with poor prognosis of CRC and circSTIL may be a potential target for CRC therapy. |
| Description: |
circSTIL is upregulated in colorectal cancer tissues and cell lines, and high expression is associated with poor prognosis. Functionally, circSTIL promotes CRC cell proliferation and inhibits ferroptosis. Mechanistically, circSTIL acts as a ceRNA for miR-431 to positively regulate SLC7A11 expression, forming a circSTIL-miR-431-SLC7A11 axis that contributes to CRC progression. |
| Confidence score: |
0.7618 |