| Expression pattern: |
UP |
| Associated gene: |
IGF1R, AGO2, PI3K, AKT, cyclinD1, p-AKT, p-PI3K |
| Associated microRNA: |
miR-502-5p |
| Biological function: |
Promotes ccRCC cell proliferation, tumor growth, infiltration, migration and metastatic capacity; knockdown suppresses these malignant phenotypes. |
| Molecular mechanism: |
hsa_circ_0003596 functions as a cytoplasmic ceRNA/miRNA sponge for miR-502-5p, thereby upregulating IGF1R and activating the downstream PI3K/AKT signaling cascade. |
| Biological pathway or process: |
PI3K/AKT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Microarray; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
hsa_circ_0003596 was associated with distant metastasis of renal cancer and may serve as a possible biomarker and therapeutic target against ccRCC. |
| Description: |
hsa_circ_0003596 is up-regulated in ccRCC tissues and cell lines and is associated with distant metastasis. Functionally, it acts as an oncogenic circRNA that promotes proliferation, tumor growth, migration, infiltration/invasion and lung metastasis. Mechanistically, cytoplasmic hsa_circ_0003596 sponges miR-502-5p to increase IGF1R expression and activate the downstream PI3K/AKT signaling cascade, suggesting potential biomarker and therapeutic-target value in ccRCC. |
| Confidence score: |
0.8748 |