circRNA basic information
circBase ID: hsa_circ_0000504
Name: hsa_circ_TUBGCP3
Synonym: circTUBGCP3
Host Gene: TUBGCP3
Genomic location(hg19): chr13:113170753-113181798:-
Genomic location(hg38): chr13:112516439-112527484:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0001056
MONDO name: gastric cancer
Disease details: gastric cancer / GC
Disease DO ID:
10534
Disease MeSH ID:
-
Disease NCIt ID:
C9331
Disease ICD11 ID:
1397617262
Disease OMIM ID:
613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

GC tissues; adjacent normal tissue samples

Cell lines:

293T; AGS; MKN28; GES-1; NCI-N87; MKN45; HGC27; HFE145

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: ENO1, GSK3beta
Associated microRNA: -
Biological function: Promotes GC cell proliferation and migration; suppresses ferroptosis; activates glycolysis; promotes tumor growth in vivo.
Molecular mechanism: circTUBGCP3 encodes TUBGCP3-230aa; the peptide binds/stabilizes ENO1 by inhibiting ubiquitination; ENO1/TUBGCP3-230aa recruits GSK3beta to suppress AMPK phosphorylation, enhancing glycolysis and inhibiting ferroptosis via system Xc-/GPX4.
Biological pathway or process:

glycolysis (promotes); ferroptosis (inhibits); proliferation (promotes); migration (promotes); ubiquitination (inhibits); AMPK (inhibits)

Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; ROC Analysis; Transfection; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; Co-IP; IP (Mass Spectrometry); Western Blot; IHC (Immunohistochemistry); Bioinformatics Analysis

Clinical significance:

ROC curve analysis suggested circTUBGCP3 expression correlates with lymph node metastasis; circTUBGCP3 may serve as a diagnostic and prognostic biomarker in GC.

Description:

circTUBGCP3 is up-regulated in gastric cancer and promotes proliferation, migration, and xenograft growth. It encodes TUBGCP3-230aa, which stabilizes ENO1 and modulates AMPK/system Xc-/GPX4 signaling to enhance glycolysis and inhibit ferroptosis.

Confidence score:

0.8768

Other information
Title:

A novel protein encoded by circTUBGCP3 blocks ferroptosis and promotes gastric cancer progression.

Journal: The Journal of biological chemistry
Published: 2025
PubMed ID: 40701248
Study type:

combined biological and clinical study

Data availability: Other data that support the findings of this study are available from the corresponding author upon reasonable request.
Code availability: -