| Expression pattern: |
UP |
| Associated gene: |
HMGA2, METTL3 |
| Associated microRNA: |
miR-133a-3p |
| Biological function: |
circ_KIAA1429 promotes HCC cell proliferation, colony formation, migration, cell mitosis and in vivo tumor growth; circ_KIAA1429 knockdown suppresses malignant phenotypes. |
| Molecular mechanism: |
METTL3-mediated m6A modification upregulates circ_KIAA1429; circ_KIAA1429 acts as a ceRNA that sponges miR-133a-3p to upregulate HMGA2, thereby promoting HCC progression. |
| Biological pathway or process: |
m6A modification (promotes); ceRNA regulation (promotes); proliferation (promotes); migration (promotes); cell cycle (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; MeRIP / MeRIP-seq; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Bioinformatics Analysis |
| Clinical significance: |
High circ_KIAA1429 expression was associated with TNM stage, tumor differentiation, tumor size and metastases stage; circ_KIAA1429 may be a potential biomarker for HCC diagnosis and treatment. |
| Description: |
circ_KIAA1429 is up-regulated in HCC tissues and cells and functions as an oncogenic circRNA. METTL3-mediated m6A modification increases circ_KIAA1429 expression, and circ_KIAA1429 promotes HCC proliferation, migration, cell-cycle-related gene expression and xenograft tumor growth by sponging miR-133a-3p to upregulate HMGA2. Its expression is associated with more advanced clinicopathologic features, suggesting potential diagnostic and therapeutic biomarker value. |
| Confidence score: |
0.8005 |