| Expression pattern: |
UP |
| Associated gene: |
UBE4A, VDAC1 |
| Associated microRNA: |
miR-326, miR-503-5p |
| Biological function: |
May promote osteosarcoma development by sponging miR-326 and miR-503-5p, thereby increasing VDAC1 and UBE4A expression. |
| Molecular mechanism: |
Predicted ceRNA mechanism: hsa_circ_0064636 sponges miR-326 and miR-503-5p to relieve repression of UBE4A and VDAC1, respectively; regulatory network not experimentally validated beyond expression RT-qPCR. |
| Biological pathway or process: |
ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
divergent primers PCR; RT-qPCR; Bioinformatics Analysis |
| Clinical significance: |
A circRNA/miRNA/mRNA interaction sub-network starting with hsa_circ_0064636 was identified with prognostic significance based on survival analysis of target genes (UBE4A, VDAC1). |
| Description: |
hsa_circ_0064636 is up-regulated in osteosarcoma cell lines. The study proposes a ceRNA sub-network in which hsa_circ_0064636 sponges miR-326 and miR-503-5p to regulate UBE4A and VDAC1, respectively, and highlights prognostic relevance through downstream target-gene survival analyses. |
| Confidence score: |
0.329 |