| Expression pattern: |
UP |
| Associated gene: |
RPN2 |
| Associated microRNA: |
miR-1258 |
| Biological function: |
promotes CRC cell proliferation, invasion, and tumor growth |
| Molecular mechanism: |
circ_SMAD2 sponges miR-1258 to upregulate RPN2 expression |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); invasion (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; divergent primers PCR; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Luciferase Reporter Assay; FISH / smFISH; Transfection; CCK8; Cell Cycle Assay; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; Western Blot; Bioinformatics Analysis; Clinical Sample Validation; Survival Analysis |
| Clinical significance: |
High circ_SMAD2 levels were positively correlated with CRC clinicopathological features; elevated circ_SMAD2 levels were correlated with advanced clinical features and poor CRC prognosis |
| Description: |
In colorectal cancer, circ_SMAD2 (hsa_circ_0000847) is up-regulated and promotes proliferation, invasion, and in vivo tumor growth. It mainly acts as a ceRNA by sponging miR-1258, thereby increasing RPN2 expression and driving CRC progression. |
| Confidence score: |
0.8539 |