| Expression pattern: |
UP |
| Associated gene: |
USP21 |
| Associated microRNA: |
hsa-miR-637 |
| Biological function: |
promotes proliferation and invasion and tumor growth in hepatocellular carcinoma |
| Molecular mechanism: |
hsa_circ_0039053 acts as a competing endogenous RNA to sponge miR-637 and thereby increases USP21 expression |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; EdU Staining; Transwell Assay; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
High hsa_circ_0039053 expression was linked to advanced TNM stage and lymph node metastasis in HCC patients |
| Description: |
In hepatocellular carcinoma, hsa_circ_0039053 (circITGAL) is up-regulated and promotes proliferation, invasion, and in vivo tumor growth. It predominantly localizes to the cytoplasm and functions as a ceRNA by sponging miR-637 to upregulate USP21. |
| Confidence score: |
0.8634 |