| Expression pattern: |
UP |
| Associated gene: |
CASP3, ZBTB20, NF-kappaB |
| Associated microRNA: |
miR-17-3p, miR-433-3p, miR-642a-5p |
| Biological function: |
Promotes macrophage apoptosis and inflammatory cytokine production, and inhibits macrophage phagocytic function in sepsis. |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA) for miR-17-3p, miR-433-3p and miR-642a-5p to regulate CASP3-mediated apoptosis and ZBTB20/NF-kappaB-mediated inflammatory cytokine secretion, affecting macrophage phagocytosis. |
| Biological pathway or process: |
apoptosis (promotes); NF-kappaB (promotes); inflammation (promotes); immune regulation (other); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; Annexin V/PI Flow Cytometry; ELISA; Luciferase Reporter Assay; Western Blot; IF (Immunofluorescence); Bioinformatics Analysis; Microarray |
| Clinical significance: |
Highly expressed in serum exosomes of sepsis patients and has potential as a diagnostic marker. |
| Description: |
hsa_circ_0001818 is up-regulated in LPS-induced septic macrophages and (previously) in serum exosomes of sepsis patients. It functions as a ceRNA to sponge miR-17-3p, miR-433-3p and miR-642a-5p, thereby promoting CASP3-associated apoptosis and enhancing inflammatory cytokine secretion via the ZBTB20/NF-kappaB axis while suppressing macrophage phagocytosis. |
| Confidence score: |
0.7204 |