| Expression pattern: |
DN |
| Associated gene: |
ETS1 |
| Associated microRNA: |
hsa-miRNA-338-3p |
| Biological function: |
hsa_circRNA_0000284 promotes EA-hy926 cell proliferation and cell cycle progression, inhibits senescence and apoptosis, and its downregulation may contribute to endothelial cell damage in CHD. |
| Molecular mechanism: |
hsa_circRNA_0000284 acts as a ceRNA by sponging hsa-miRNA-338-3p and regulating the downstream target gene ETS1, with AluSq2 affecting circRNA formation and expression. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); ceRNA regulation (other); other pathway/process (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; Sanger Sequencing |
| Clinical significance: |
hsa_circRNA_0000284 is downregulated in PBLs from CHD patients and may be associated with CHD pathogenesis and potential diagnosis or treatment targets. |
| Description: |
In CHD, hsa_circRNA_0000284 is downregulated in peripheral blood leukocytes. Functionally, hsa_circRNA_0000284 supports endothelial cell proliferation and cell cycle progression while suppressing senescence and apoptosis. Mechanistically, it acts as a ceRNA for hsa-miRNA-338-3p to regulate ETS1 expression, suggesting a role for the hsa_circRNA_0000284/hsa-miRNA-338-3p/ETS1 axis in CHD pathogenesis. |
| Confidence score: |
0.7404 |