| Expression pattern: |
UP |
| Associated gene: |
COPB1, SP1 |
| Associated microRNA: |
- |
| Biological function: |
Promotes TNBC cell proliferation, cell cycle progression and xenograft tumor growth; inhibits apoptosis. |
| Molecular mechanism: |
SP1 binds the SNX25 promoter to enhance circSNX25 biogenesis, and circSNX25 physically interacts with COPB1 in TNBC. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); cell cycle (promotes); other pathway/process (other) |
| Detected method: |
Q
S
|
| Validation methods: |
Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; TUNEL; In Vivo Animal Model; H&E Staining; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
CircSNX25 may serve as a diagnostic and therapeutic biomarker, a prognostic marker, and a therapeutic target for TNBC. |
| Description: |
circSNX25 is an up-regulated circular RNA in TNBC tissues and cells. It promotes TNBC proliferation, cell cycle progression and xenograft tumor growth while suppressing apoptosis. Mechanistically, SP1 enhances circSNX25 biogenesis through the SNX25 promoter, and circSNX25 physically interacts with COPB1, supporting its potential value as a TNBC biomarker and therapeutic target. |
| Confidence score: |
0.8574 |