circRNA basic information
circBase ID: hsa_circ_0000043
Name: hsa_circ_PUM1
Synonym: -
Host Gene: PUM1
Genomic location(hg19): chr1:31465236-31468067:-
Genomic location(hg38): chr1:30992389-30995220:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008903
MONDO name: lung cancer
Disease details: lung cancer
Disease DO ID:
 1324
Disease MeSH ID:
 -
Disease NCIt ID:
 C7377
Disease ICD11 ID:
 -
Disease OMIM ID:
 211980
Species: Human
Species details: Homo sapiens
Tissue specimen:

lung cancer tissue; paired adjacent normal tissue; matched adjacent healthy tissues
Cell lines:

16HBE; 16HBE-T; A549; H1299; H446; 95D
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: BDNF, STAT3
Associated microRNA: miR-4492
Biological function: Promotes lung cancer cell proliferation, malignant transformation ability, migration, invasion, metastasis-related progression, and tumor growth; inhibition of hsa_circ_0000043 suppresses these phenotypes.
Molecular mechanism: hsa_circ_0000043 acts as a miR-4492 sponge and regulates BDNF and STAT3 expression via miR-4492, functioning as a competitive endogenous RNA mechanism.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); other pathway/process (promotes)
Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; Microarray; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; Western Blot; Survival Analysis; Bioinformatics Analysis
Clinical significance:

hsa_circ_0000043 overexpression is associated with TNM stage, distant metastasis, lymph-node metastasis, smoking exposure, and poor overall survival; it may serve as a diagnostic marker and therapeutic target for lung cancer.
Description:

hsa_circ_0000043 is up-regulated in BPDE-transformed 16HBE-T cells, lung cancer cell lines, and lung cancer tissues. It promotes lung cancer progression by sponging miR-4492 and regulating BDNF and STAT3 expression, thereby enhancing proliferation, malignant transformation, migration, invasion, and xenograft tumor growth. Clinically, high hsa_circ_0000043 expression is associated with advanced TNM stage, metastasis, smoking exposure, and poor overall survival.
Confidence score:

0.8175
Other information
Title:

CircRNA hsa_circ_0000043 acts as a miR-4492 sponge to promote lung cancer progression via BDNF and STAT3 expression regulation in anti-benzo[a]pyrene-trans-7,8-dihydrodiol-9,10-epoxide-transformed 16HBE cells.

Journal: Toxicological sciences : an official journal of the Society of Toxicology
Published: 2023
PubMed ID: 37326964
Study type:

combined biological and clinical study
Data availability: TCGA database; Supplementary data are available at Toxicological Sciences online
Code availability: -