| Expression pattern: |
UP |
| Associated gene: |
ERalpha, SMG1, GSN, GSN-mRNA, Argonaute 2 |
| Associated microRNA: |
miR-141-3p |
| Biological function: |
circRNA-SMG1.72 promotes HCC cell invasion and metastasis; knockdown of circRNA-SMG1.72 decreases GSN expression and suppresses invasion, while overexpression increases GSN expression and invasion. |
| Molecular mechanism: |
ERalpha transcriptionally suppresses circRNA-SMG1.72 expression by binding the SMG1 promoter; circRNA-SMG1.72 acts as a ceRNA sponge for miR-141-3p, relieving miR-141-3p-mediated repression of GSN mRNA translation and thereby increasing GSN expression. |
| Biological pathway or process: |
invasion (promotes); metastasis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Elevated circSMG1.72 in HCC patients may contribute to HCC invasion through the miR-141-3p/GSN axis, and targeting circ-SMG1.72 may be a potential treatment strategy for HCC, especially ER-low tumors. |
| Description: |
In HCC, circRNA-SMG1.72 is elevated and functions as a pro-invasive circRNA. ERalpha suppresses circRNA-SMG1.72 by transcriptional regulation of its host gene SMG1, while circRNA-SMG1.72 sponges miR-141-3p to relieve repression of GSN and promote HCC invasion and metastasis. Targeting the ERalpha/circRNA-SMG1.72/miR-141-3p/GSN axis may help suppress HCC progression, especially in ER-low tumors. |
| Confidence score: |
0.8468 |