circRNA basic information
circBase ID: hsa_circ_0080220
Name: hsa_circ_EGFR
Synonym: CircEGFR
Host Gene: EGFR
Genomic location(hg19): chr7:55209978-55214433:+
Genomic location(hg38): chr7:55142285-55146740:+
Subcellular localization: cytoplasm; exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005494
MONDO name: triple-negative breast carcinoma
Disease details: triple-negative breast cancer / TNBC
Disease DO ID:
 0060081
Disease MeSH ID:
 D064726
Disease NCIt ID:
 C71732
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

TNBC tissues; paired normal tissues; adjacent normal tissues; plasma exosomes; xenograft tumors
Cell lines:

MCF-10A; MCF-7; BT-474; SKBR-3; HCC-1806; MDA-MB-231; MDA-MB-486; MDA-MB-468
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: EGFR
Associated microRNA: miR-1299
Biological function: Promotes proliferation, migration, invasion, EMT, tumor growth and THP chemotherapy resistance in TNBC; reduces sensitivity to THP treatment.
Molecular mechanism: circEGFR acts as a competing endogenous RNA that sponges miR-1299 to regulate EGFR expression through the circEGFR/miR-1299/EGFR axis.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); metastasis (promotes); ceRNA regulation (promotes); chemoresistance (promotes); drug resistance (promotes)
Detected method:
Q
S
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Wound Healing Assay; Transwell Assay; Western Blot; IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Highly expressed in TNBC tissues and plasma exosomes; associated with poor prognosis; potential diagnostic, therapeutic and prognostic biomarker for TNBC.
Description:

circEGFR (hsa_circ_0080220) is up-regulated in TNBC tissues, TNBC cell lines and plasma exosomes, and high expression is associated with poor prognosis. It promotes TNBC proliferation, migration, invasion, EMT, xenograft tumor growth and THP chemoresistance by sponging miR-1299 and increasing EGFR expression. The study suggests circEGFR is a potential diagnostic, prognostic and therapeutic biomarker for TNBC.
Confidence score:

0.8705
Other information
Title:

CircEGFR reduces the sensitivity of pirarubicin and regulates the malignant progression of triple-negative breast cancer via the miR-1299/EGFR axis.

Journal: International journal of biological macromolecules
Published: 2023
PubMed ID: 37302631
Study type:

combined biological and clinical study
Data availability: NCBI SRP266211; https://doi. org/10.1016/j.ijbiomac.2023.125295; Additional data pertaining to this work can be obtained from the corresponding author upon request.
Code availability: -