| Expression pattern: |
UP |
| Associated gene: |
SIX1, FOXC2, CASK promoter, Wnt/beta-catenin pathway |
| Associated microRNA: |
miR-1271-5p |
| Biological function: |
circCASK promotes CRC cell growth, proliferation, migration, invasion, metastasis-related abilities, tumorigenesis, and xenograft tumor growth. |
| Molecular mechanism: |
FOXC2 transcriptionally induces circCASK expression; cytoplasmic circCASK acts as a miRNA sponge for miR-1271-5p, upregulates SIX1, and activates Wnt/beta-catenin signaling. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); Wnt/beta-catenin (promotes); ceRNA regulation (other) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Luciferase Reporter Assay; Western Blot; ChIP / ChIP-seq; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
circCASK is highly expressed in clinical CRC samples and may act as a potential biomarker and therapeutic target; circCASK expression correlates with poor survival of CRC patients. |
| Description: |
circCASK (hsa_circ_0001917), derived from the CASK gene, is upregulated in CRC tissues and cell lines. FOXC2 induces circCASK transcription, and cytoplasmic circCASK promotes CRC proliferation, migration, invasion, and xenograft tumor growth by sponging miR-1271-5p, increasing SIX1 expression, and activating Wnt/beta-catenin and EMT-related programs. The study suggests circCASK has potential biomarker and therapeutic-target value in CRC. |
| Confidence score: |
0.8571 |