| Expression pattern: |
UP |
| Associated gene: |
NUMB, NOTCH3, HES1, GATAD2A |
| Associated microRNA: |
miR-218-5p, miR-665 |
| Biological function: |
promotes PTC cell proliferation, tumorigenicity, migration, invasion, and metastasis; increases cell viability; inhibits apoptosis |
| Molecular mechanism: |
ceRNA mechanism: hsa_circ_0058124 sponges miR-218-5p to modulate NUMB and suppress the NOTCH3/GATAD2A axis |
| Biological pathway or process: |
Notch (inhibits); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; RNase R Treatment; divergent primers PCR; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; RNA-seq; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
hsa_circ_0058124 upregulation is correlated with unfavorable prognosis and is a potential prognostic biomarker in PTC |
| Description: |
hsa_circ_0058124 is up-regulated in papillary thyroid cancer and correlates with unfavorable prognosis. It promotes proliferation and malignant behaviors by acting as a ceRNA (sponging miR-218-5p) to increase NUMB and thereby suppress the NOTCH3/GATAD2A axis, validated in vitro and in a xenograft model. |
| Confidence score: |
0.8442 |