circRNA basic information
circBase ID: hsa_circ_0000735
Name: -
Synonym: circ_0000735
Host Gene: -
Genomic location(hg19): chr17:3802927-3808661:-
Genomic location(hg38): chr17:3899633-3905367:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissue samples; adjacent normal tissue; tumor samples; normal tissues
Cell lines:

A549; H358; H1299; H1581; 16HBE
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: -
Associated microRNA: miR-1179, miR-1182
Biological function: circ_0000735 functions as an oncogene in NSCLC cells, promoting proliferation, colony formation, migration and invasion while inhibiting apoptosis.
Molecular mechanism: circ_0000735 acts as a miRNA sponge for miR-1179 and miR-1182; its oncogenic effects are partly dependent on regulation of miR-1179/1182.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; Bioinformatics Analysis
Clinical significance:

Upregulation of circ_0000735 is associated with more advanced TNM stages and lymph node invasion/metastasis in NSCLC patients.
Description:

circ_0000735 is up-regulated in NSCLC tissues and cell lines, and its higher expression is associated with advanced TNM stage and lymph node metastasis. Functionally, it promotes NSCLC cell proliferation, colony formation, migration and invasion and suppresses apoptosis. Mechanistically, circ_0000735 acts as a sponge for miR-1179 and miR-1182, although downstream regulators were not identified.
Confidence score:

0.6521
Other information
Title:

Enhanced expression of circ_0000735 forecasts clinical severity in NSCLC and promotes cell progression via sponging miR-1179 and miR-1182.

Journal: Biochemical and biophysical research communications
Published: 2019
PubMed ID: 30737027
Study type:

combined biological and clinical study
Data availability: -
Code availability: -