| Expression pattern: |
UP |
| Associated gene: |
GBP1, Ago2 |
| Associated microRNA: |
miR-1299 |
| Biological function: |
promotes NSCLC cell proliferation, migration, invasion, EMT, tumor growth and taxol resistance; inhibits taxol-induced apoptosis |
| Molecular mechanism: |
circ_0058608 acts as a miR-1299 sponge to positively regulate GBP1 expression, thereby promoting NSCLC progression and taxol resistance |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); apoptosis (inhibits); ceRNA regulation (promotes); drug resistance (promotes); chemoresistance (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; EdU Staining; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); CCK8; Annexin V/PI Flow Cytometry; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Survival Analysis |
| Clinical significance: |
high circ_0058608 expression is associated with poor survival in NSCLC patients and circ_0058608 siRNA might be a possible therapeutic approach for NSCLC tumorigenesis and drug resistance |
| Description: |
circ_0058608 is up-regulated in NSCLC and further elevated in taxol-resistant NSCLC tissues and cells. It promotes NSCLC proliferation, migration, invasion, EMT, tumor growth, and taxol resistance by sponging miR-1299 and upregulating GBP1; high expression is associated with poor patient survival. |
| Confidence score: |
0.7794 |