| Expression pattern: |
UP |
| Associated gene: |
E2F1 |
| Associated microRNA: |
miR-1205 |
| Biological function: |
promotes proliferation and migration of HCC cells |
| Molecular mechanism: |
ceRNA mechanism: circMYBL2 sponges miR-1205 to increase E2F1 expression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
potential of circMYBL2 as a novel target for HCC treatment or a prognostic biomarker for HCC |
| Description: |
circMYBL2 is up-regulated in HCC tissues and cell lines and promotes HCC cell proliferation and migration. It functions as a ceRNA by sponging miR-1205, thereby increasing E2F1 expression and accelerating HCC progression, and is proposed as a potential therapeutic target/prognostic biomarker. |
| Confidence score: |
0.8487 |