| Expression pattern: |
UP |
| Associated gene: |
HIF1alpha, VEGFA |
| Associated microRNA: |
- |
| Biological function: |
inhibits endothelial angiogenic functions (proliferation, migration, tube formation) and reduces blood flow recovery after ischemia |
| Molecular mechanism: |
Exosomal cZFP609 is delivered from VSMCs to ECs and binds HIF1alpha, sequestering it in the cytoplasm to block HIF1alpha nuclear translocation, thereby inhibiting HIF1alpha-driven VEGFA transcription/expression under hypoxia. |
| Biological pathway or process: |
angiogenesis (inhibits); proliferation (inhibits); migration (inhibits); HIF-1 signaling (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; BrdU; Wound Healing Assay; Tube Formation Assay; In Vivo Animal Model; Western Blot; IF (Immunofluorescence); H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
Plasma cZNF609 is higher in patients with atherosclerotic or diabetic lower extremity PAD and is associated with reduced ankle-brachial index (ABI), suggesting potential value for assessing severity/blood flow perfusion. |
| Description: |
cZFP609/cZNF609 is increased (notably in VSMC-derived exosomes and in patient plasma) in ischemia/PAD settings and suppresses angiogenesis. It binds HIF1alpha in EC cytoplasm to block nuclear translocation and downstream VEGFA induction, thereby inhibiting EC proliferation, migration, and tube formation; its knockdown improves perfusion recovery in hindlimb ischemia models. |
| Confidence score: |
0.6744 |