| Expression pattern: |
UP |
| Associated gene: |
PRC1, Ago2 |
| Associated microRNA: |
miR-1322 |
| Biological function: |
circKDM1B promotes HCC cell proliferation, migration, invasion and tumor growth, and inhibits apoptosis. |
| Molecular mechanism: |
circKDM1B acts as a ceRNA sponge for miR-1322 to upregulate PRC1 expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; Nuclear-Cytoplasmic Fractionation; IHC (Immunohistochemistry); In Vivo Animal Model; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circKDM1B expression was associated with TNM stage and poor 5-year overall survival of HCC patients, suggesting potential prognostic and therapeutic value. |
| Description: |
circKDM1B is up-regulated in HCC tissues and cells and is associated with advanced tumor stage and poor overall survival. It promotes HCC progression by enhancing proliferation, migration, invasion and in vivo tumor growth while suppressing apoptosis. Mechanistically, circKDM1B acts as a miR-1322 sponge to increase PRC1 expression, forming the circKDM1B/miR-1322/PRC1 regulatory axis. |
| Confidence score: |
0.8557 |