| Expression pattern: |
DS |
| Associated gene: |
- |
| Associated microRNA: |
- |
| Biological function: |
CircMTO1 was used as the target circRNA for an ultrasensitive biosensor and its abnormal expression was used to discriminate cancer cells and breast cancer patient tissues from controls. |
| Molecular mechanism: |
The study focuses on a detection mechanism in which circMTO1 triggers proximity ligation-activated recombinase polymerase amplification followed by transcription amplification to generate Spinach RNA aptamers and DFHBI fluorescence; no endogenous disease-regulatory molecular mechanism was tested. |
| Biological pathway or process: |
not specified |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation |
| Clinical significance: |
The dysregulation of circMTO1 may act as a potential prognostic marker for breast cancer, and the biosensor can discriminate circMTO1 levels between breast cancer patient tissues and healthy tissues. |
| Description: |
This study used circMTO1 as the model target to develop and validate a label-free ultrasensitive fluorescent biosensor. Endogenous circMTO1 was dysregulated across tested human cancer cell lines and was down-regulated in breast cancer patient tissues compared with healthy tissues, supporting its potential clinical utility as a cancer-related biomarker. |
| Confidence score: |
0.2995 |