circRNA basic information
circBase ID: hsa_circ_0005239
Name: hsa_circ_GFRA1
Synonym: circGFRA1
Host Gene: GFRA1
Genomic location(hg19): chr10:117849251-117856275:-
Genomic location(hg38): chr10:116089740-116096764:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tumor samples; paired noncancerous liver tissues; adjacent nontumor liver tissues
Cell lines:

LO2; HepG2; MHCC-97H; SMMC-7721; Bel-7402; MHCC-97L; HUVECs
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PD-L1, p-Akt, AKT, CD31
Associated microRNA: miR-34a-5p / miR-34a
Biological function: Promotes HCC cell migration, invasion, angiogenesis, xenograft tumor growth, and malignant progression; knockdown suppresses these phenotypes.
Molecular mechanism: hsa_circ_0005239 acts as a competing endogenous RNA and sponge for miR-34a-5p, relieving miR-34a-5p-mediated repression of PD-L1 and activating the PI3K/Akt signaling pathway.
Biological pathway or process:

PI3K/AKT (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); ceRNA regulation (promotes); proliferation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; FISH / smFISH; Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; Transwell Assay; Tube Formation Assay; Western Blot; In Vivo Animal Model
Clinical significance:

potential diagnostic biomarker and therapeutic target for HCC
Description:

hsa_circ_0005239 is upregulated in HCC tissues and cell lines and functions as an oncogenic circRNA. It promotes HCC migration, invasion, angiogenesis, and xenograft growth by sponging miR-34a-5p, increasing PD-L1 expression, and activating the PI3K/Akt pathway. The study suggests hsa_circ_0005239 may serve as a potential diagnostic biomarker and therapeutic target for HCC.
Confidence score:

0.6815
Other information
Title:

Circular RNA hsa_circ_0005239 contributes to hepatocellular carcinoma cell migration, invasion, and angiogenesis by targeting the miR-34a-5p/PD-L1 axis.

Journal: Cell biology international
Published: 2023
PubMed ID: 37208963
Study type:

combined biological and clinical study
Data availability: The data used to support the findings of this study are available from the corresponding author upon request.
Code availability: -