circRNA basic information
circBase ID: hsa_circ_0066436
Name: hsa_circ_ATXN7
Synonym: circATXN7 / circ_0066436
Host Gene: ATXN7
Genomic location(hg19): chr3:63973734-63976535:+
Genomic location(hg38): chr3:63988058-63990859:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer / BC
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

Tumor tissue samples; matched normal samples; breast cancer tissues; normal tissues
Cell lines:

MCF10A; MCF7; T47D; MDA-MB-231; MDA-MB-468; BT549
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: HOXA11
Associated microRNA: miR-149-5p
Biological function: CircATXN7 promotes breast cancer progression, cell proliferation, migration, invasion, and doxorubicin resistance; circATXN7 silencing suppresses tumor progression and enhances doxorubicin sensitivity.
Molecular mechanism: CircATXN7 acts as a ceRNA by sponging miR-149-5p to upregulate HOXA11, thereby promoting breast cancer progression and doxorubicin resistance.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); drug resistance (promotes); chemoresistance (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circATXN7 expression is associated with lymph node metastasis, higher TNM stage, doxorubicin resistance, and poor overall survival, and may provide a possible biomarker for breast cancer therapy.
Description:

CircATXN7 is up-regulated in breast cancer tissues and cells and its high expression is associated with lymph node metastasis, advanced stage, doxorubicin resistance, and poor overall survival. Functionally, circATXN7 promotes proliferation, migration, invasion, tumor growth, and doxorubicin resistance. Mechanistically, circATXN7 sponges miR-149-5p to increase HOXA11 expression, forming a circATXN7/miR-149-5p/HOXA11 axis that drives breast cancer progression and chemoresistance.
Confidence score:

0.8146
Other information
Title:

CircATXN7 contributes to the progression and doxorubicin resistance of breast cancer via modulating miR-149-5p/HOXA11 pathway.

Journal: Anti-cancer drugs
Published: 2022
PubMed ID: 34845164
Study type:

combined biological and clinical study
Data availability: The analyzed data sets generated during the present study are available from the corresponding author on reasonable request.
Code availability: -