| Expression pattern: |
DN |
| Associated gene: |
LINC00632, IGF2BP2, IGF2BP3, SNAI2, MEF2C, NTRK2, SEMA6D |
| Associated microRNA: |
miR-7 / miR-7-5p, miR-671-5p |
| Biological function: |
Suppresses melanoma invasion in vitro and metastasis in vivo; CDR1as depletion promotes invasion and lung metastasis. |
| Molecular mechanism: |
Epigenetic silencing of the LINC00632/CDR1as locus by PRC2/H3K27me3; miR-7-independent mechanism involving interaction with IGF2BP3 and IGF2BP3-dependent pro-invasive targets. |
| Biological pathway or process: |
invasion (promotes); metastasis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; FISH / smFISH; Clinical Sample Validation; Survival Analysis; Transfection; Transwell Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Low CDR1as abundance associates with shorter progression-free and overall survival in primary melanoma patient samples; CDR1as may have prognostic value. |
| Description: |
In melanoma, CDR1as is epigenetically silenced during progression and functions as a metastasis suppressor. Loss of CDR1as promotes invasion and lung metastasis via a miR-7-independent mechanism that requires interaction with the RBP IGF2BP3 and regulation of pro-invasive IGF2BP3 targets. |
| Confidence score: |
0.8222 |