circRNA basic information
circBase ID: hsa_circ_0007552
Name: hsa_circ_RILPL1
Synonym: circRILPL1
Host Gene: RILPL1
Genomic location(hg19): chr12:123983090-123984083:-
Genomic location(hg38): chr12:123498543-123499536:-
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0015459
MONDO name: nasopharyngeal carcinoma
Disease details: nasopharyngeal carcinoma / NPC
Disease DO ID:
 9261
Disease MeSH ID:
 D00007727; D000077274
Disease NCIt ID:
 C3871
Disease ICD11 ID:
 1883313543
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NPC tissues; chronic rhinitis epithelial tissues; non-cancer nasopharyngeal epithelial tissues; adjacent non-NPC tissues; lung tissues; inguinal lymph nodes
Cell lines:

HNE2; CNE2; HONE1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ROCK1, IPO7, YAP, LATS1, CAPN2, PXN
Associated microRNA: -
Biological function: circRILPL1 promotes NPC cell proliferation, migration, invasion, lung and lymph node metastasis, weakens adhesion, decreases stiffness, promotes actin filament polymerization, and contributes to malignant progression.
Molecular mechanism: circRILPL1 binds to and activates ROCK1 to inhibit the LATS1-YAP kinase cascade, reducing YAP phosphorylation and ubiquitin-mediated degradation; it also binds and cooperates with IPO7 to enhance YAP nuclear translocation, thereby activating YAP-mediated transcription of CAPN2 and PXN through the Hippo-YAP signaling pathway.
Biological pathway or process:

Hippo/YAP (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ubiquitination (inhibits); other pathway/process (promotes)
Detected method:
Q
H
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; ISH (In Situ Hybridization); FISH / smFISH; IHC (Immunohistochemistry); IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; Western Blot; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Highly expressed circRILPL1 in NPC may serve as an important biomarker for tumor diagnosis and may also be a potential therapeutic target; high circRILPL1 expression is associated with lower overall survival in NPC patients.
Description:

circRILPL1 is up-regulated in nasopharyngeal carcinoma and high expression is associated with poorer overall survival. Functionally, it promotes NPC proliferation, invasion, migration, and metastasis in vitro and in vivo. Mechanistically, circRILPL1 binds ROCK1 and IPO7 to activate Hippo-YAP signaling, stabilize and nuclear-translocate YAP, and enhance CAPN2 and PXN transcription.
Confidence score:

0.8995
Other information
Title:

Circular RNA circRILPL1 promotes nasopharyngeal carcinoma malignant progression by activating the Hippo-YAP signaling pathway.

Journal: Cell death and differentiation
Published: 2023
PubMed ID: 37173390
Study type:

combined biological and clinical study
Data availability: GSE68799; https://doi.org/10.1038/s41418-023-01171-8; All data that support the findings of this study are available from the corresponding authors upon reasonable request.
Code availability: -