circRNA basic information
circBase ID: hsa_circ_0000523
Name: hsa_circ_METTL3
Synonym: circ_0000523 / circ6229
Host Gene: METTL3
Genomic location(hg19): chr14:21971315-21972024:-
Genomic location(hg38): chr14:21503172-21503881:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

HCT116
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UN
Associated gene: METTL3
Associated microRNA: miR-let-7b
Biological function: Promotes proliferation and invasion and inhibits apoptosis in HCT116 colorectal cancer cells.
Molecular mechanism: hsa_circ_0000523 suppresses miR-let-7b, thereby upregulating METTL3 (ceRNA-like regulation).
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); invasion (promotes); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot
Clinical significance:

circRNAs and METTL3 may be applied for highly sensitive diagnosis of CRC and for predicting prognosis in patients who have undergone therapy.
Description:

In HCT116 colorectal cancer cells, hsa_circ_0000523 promotes proliferation and invasion and inhibits apoptosis. Mechanistically, it forms a hsa_circ_0000523/miR-let-7b/METTL3 regulatory axis in which circ_0000523 suppresses miR-let-7b and thereby increases METTL3 expression.
Confidence score:

0.528
Other information
Title:

hsa_circ_0000523/miR-let-7b/METTL3 axis regulates proliferation, apoptosis and metastasis in the HCT116 human colorectal cancer cell line.

Journal: Oncology letters
Published: 2022
PubMed ID: 35527788
Study type:

biological research
Data availability: The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -