| Expression pattern: |
UP |
| Associated gene: |
CCNE1, Ago2 |
| Associated microRNA: |
miR-195-5p |
| Biological function: |
promotes CRC cell proliferation, migration, invasion, malignant phenotype, tumorigenesis and tumor growth; circSEMA5A silencing represses tumor metastasis and reduces xenograft tumor growth |
| Molecular mechanism: |
circSEMA5A acts as a ceRNA/miRNA sponge by competitively binding miR-195-5p, thereby upregulating CCNE1 expression |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); cell cycle (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Transfection; Colony Formation Assay; CCK8; Transwell Assay; Wound Healing Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis |
| Clinical significance: |
providing a new direction for CRC diagnosis and targeted therapy; potential new direction for seeking therapeutic plans to treat CRC |
| Description: |
circSEMA5A is up-regulated in CRC cells and functions as an oncogenic circRNA. It promotes proliferation, migration, invasion and xenograft tumor growth by sponging miR-195-5p and increasing CCNE1 expression, suggesting potential relevance for CRC targeted therapy. |
| Confidence score: |
0.8354 |