| Expression pattern: |
UP |
| Associated gene: |
CDK6, AGO2, METTL9, Ki67 |
| Associated microRNA: |
miR-551b-5p |
| Biological function: |
Promotes colorectal cancer cell proliferation, migration, invasion, tumor growth and metastasis; accelerates cell cycle progression. |
| Molecular mechanism: |
circ-METTL9 acts as a miRNA sponge for miR-551b-5p and upregulates CDK6, thereby promoting colorectal cancer progression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); cell cycle (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); circRIP; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Survival Analysis; Bioinformatics Analysis; RNA-seq |
| Clinical significance: |
High circ-METTL9 expression is associated with advanced CRC stages and shorter overall survival; it may serve as a prognostic biomarker and therapeutic target. |
| Description: |
circ-METTL9 is a METTL9-derived circular RNA upregulated in colorectal cancer tissues and associated with advanced disease and shorter overall survival. Functionally, it promotes CRC proliferation, migration, invasion, tumor growth and metastasis. Mechanistically, circ-METTL9 acts as a miR-551b-5p sponge to increase CDK6 expression through the circ-METTL9/miR-551b-5p/CDK6 axis. |
| Confidence score: |
0.8904 |