| Expression pattern: |
UP |
| Associated gene: |
AGO2, FAK |
| Associated microRNA: |
miR-1224 |
| Biological function: |
promotes proliferation, migration, invasion, EMT, tumor growth and metastasis; inhibits apoptosis; promotes cell cycle progression |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA) for miR-1224 to upregulate FAK |
| Biological pathway or process: |
ceRNA regulation (promotes); focal adhesion (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); apoptosis (inhibits); cell cycle (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; TUNEL; Transwell Assay; Western Blot; RIP (RNA Immunoprecipitation); circRIP; RNA Pull-Down; Luciferase Reporter Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation; Cohort Study; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
AUC for distinguishing HCC tissues from ANL tissues was 0.882; high circRASGRF2 correlated with poorer differentiation, advanced stage, larger tumor size, microvascular invasion, and shorter overall survival |
| Description: |
circRASGRF2 (hsa_circ_0073181) is upregulated in HCC and primarily localized in the cytoplasm. It promotes HCC cell proliferation, migration/invasion, EMT, and tumor growth/metastasis by sponging miR-1224 to derepress FAK, and high expression is associated with poor overall survival and diagnostic value (AUC 0.882). |
| Confidence score: |
0.898 |