circRNA basic information
circBase ID: hsa_circ_0086996
Name: hsa_circ_RECK
Synonym: Circular RNA 0086996
Host Gene: RECK
Genomic location(hg19): chr9:36066765-36112473:+
Genomic location(hg38): chr9:36066768-36112476:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

osteosarcoma tissue; paired adjacent noncancerous tissue; tumor tissue; adjacent tissue
Cell lines:

OB3; U2-OS; SAOS2; HOS; 143B; MG-63
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: -
Associated microRNA: miR-125b-5p
Biological function: Circ_0086996 promotes osteosarcoma cell proliferation, migration, and invasion, inhibits apoptosis, and supports cell cycle progression; knockdown suppresses proliferation, migration and invasion, induces G0/G1 cell cycle arrest, and promotes apoptosis.
Molecular mechanism: Circ_0086996 acts as a sponge for miR-125b-5p and mediates osteosarcoma tumorigenicity by inhibiting miR-125b-5p activity.
Biological pathway or process:

ceRNA regulation (other); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; Luciferase Reporter Assay; Bioinformatics Analysis
Clinical significance:

Circ_0086996 could act as a potential biomarker for osteosarcoma.
Description:

hsa_circ_0086996/Circ_0086996 is up-regulated in osteosarcoma tissues and cell lines. It functions as an oncogenic circRNA by sponging miR-125b-5p, thereby promoting proliferation, migration, invasion and cell cycle progression while inhibiting apoptosis. The study suggests Circ_0086996 may serve as a potential biomarker and therapeutic target for osteosarcoma.
Confidence score:

0.6536
Other information
Title:

Circular RNA 0086996 regulates growth and migration of osteosarcoma cells via miR-125b-5p.

Journal: Pathology, research and practice
Published: 2020
PubMed ID: 33053498
Study type:

combined biological and clinical study
Data availability: The datasets supporting the conclusions of this article are included within the article.
Code availability: -