| Expression pattern: |
UP |
| Associated gene: |
beta-catenin |
| Associated microRNA: |
- |
| Biological function: |
Promotes CRC cell stemness, sphere formation, migration, metastasis and tumorigenesis. |
| Molecular mechanism: |
circ-ABCC1 binds beta-catenin, promotes beta-catenin nuclear accumulation, and activates the Wnt/beta-catenin pathway; exosomes from CD133+ cells carry circ-ABCC1. |
| Biological pathway or process: |
Wnt/beta-catenin (promotes); stemness (promotes); migration (promotes); metastasis (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Bioinformatics Analysis; Back-Splice Junction PCR / divergent primers PCR; Actinomycin D / DRB Stability Assay; RNase R Treatment; RT-qPCR; Transfection; Flow Cytometry（Non-apoptosis/cycle）; Transwell Assay; Western Blot; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down |
| Clinical significance: |
circ-ABCC1 serves as a novel candidate target for CRC treatment and may be utilized as a biomarker in CRC research works. |
| Description: |
This study identifies hsa_circ_0000677 (circ-ABCC1) as an upregulated exosomal circRNA in CRC. circ-ABCC1 promotes CRC cell stemness, sphere formation, migration and metastasis, and mechanistically binds beta-catenin to enhance its nuclear accumulation and activate the Wnt/beta-catenin pathway. The authors propose circ-ABCC1 as a potential therapeutic target or biomarker candidate for CRC research. |
| Confidence score: |
0.7884 |