circRNA basic information
circBase ID: -
Name: hsa_circ_KIF4A
Synonym: Circular RNA KIF4A / circKIF4A
Host Gene: KIF4A
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005494
MONDO name: triple-negative breast carcinoma
Disease details: Triple-negative breast cancer / TNBC
Disease DO ID:
 0060081
Disease MeSH ID:
 D064726
Disease NCIt ID:
 C71732
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; paired adjacent normal tissues; tumor tissues and lung tissues
Cell lines:

MCF10A; BT549; HCC38; MDA-MB-453; MDA-MB-468; MDA-MB-231
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: EIF4A3, SDC1, c-src, FAK
Associated microRNA: -
Biological function: Promotes TNBC cell proliferation, viability, migration, invasion, EMT-associated changes, tumor growth, and TNBC progression.
Molecular mechanism: circKIF4A recruits and binds the RNA-binding protein EIF4A3, enhances SDC1 mRNA stability and SDC1 expression, and activates c-src/FAK signaling pathways to promote TNBC progression.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); mRNA stability (promotes); other pathway/process (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Actinomycin D / DRB Stability Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model
Clinical significance:

May provide a potential therapeutic target for TNBC treatment.
Description:

circKIF4A is up-regulated in TNBC tissues and cell lines and acts as an oncogenic circRNA. It binds EIF4A3 to stabilize SDC1 mRNA, thereby increasing SDC1 expression and activating c-src/FAK signaling to promote TNBC cell proliferation, migration, invasion, EMT-related changes, and xenograft tumor growth.
Confidence score:

0.8252
Other information
Title:

CircKIF4A combines EIF4A3 to stabilize SDC1 expression to activate c-src/FAK and promotes TNBC progression.

Journal: Cellular signalling
Published: 2023
PubMed ID: 37121557
Study type:

combined biological and clinical study
Data availability: The datasets generated during and/or analyzed during the current study are available from the corresponding author upon reasonable request.
Code availability: -