| Expression pattern: |
UP |
| Associated gene: |
TOP2A / DNA topoisomerase 2-alpha, UBAP2 |
| Associated microRNA: |
miR-496 |
| Biological function: |
Promotes bladder cancer cell growth, proliferation, migration, invasion, aerobic glycolysis, and tumor growth in vivo; inhibits apoptosis. Knockdown suppresses these oncogenic phenotypes and induces apoptosis. |
| Molecular mechanism: |
circUBAP2 acts as a ceRNA sponge for miR-496, relieving miR-496-mediated repression of TOP2A and thereby indirectly regulating TOP2A expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); glycolysis (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; Colony Formation Assay; EdU Staining; Transwell Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circUBAP2 expression was associated with advanced T grade, lymph node metastasis, bigger tumor size, and poor overall survival in bladder cancer patients. |
| Description: |
circUBAP2 is up-regulated in bladder cancer and high expression is associated with poor overall survival and aggressive clinicopathological features. Functionally, circUBAP2 promotes BC cell proliferation, migration, invasion, aerobic glycolysis, and xenograft tumor growth while inhibiting apoptosis. Mechanistically, circUBAP2 acts as a ceRNA for miR-496 to up-regulate TOP2A. |
| Confidence score: |
0.8202 |