| Expression pattern: |
UP |
| Associated gene: |
PTBP1, GLIS3 pre-mRNA intron 4, GLIS3 pre-mRNA intron 8 |
| Associated microRNA: |
miR-526b, miR-198, miR-498, miR-664a, miR-644a |
| Biological function: |
Promotes proliferation, migration, invasion, tumor growth and metastasis, and inhibits apoptosis of NSCLC cells. |
| Molecular mechanism: |
circGLIS3 acts as a miRNA sponge for multiple tumor-suppressive miRNAs, especially miR-644a, upregulates PTBP1 via miR-644a, and PTBP1 promotes circGLIS3 circularization by binding flanking introns, forming a circGLIS3/miR-644a/PTBP1 positive feedback loop. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); metastasis (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; Microarray; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; Western Blot; IF (Immunofluorescence); Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circGLIS3 level was correlated with malignant characteristics and poor prognosis of NSCLC; it might be a potential biomarker for NSCLC. |
| Description: |
circGLIS3 is an up-regulated circRNA in NSCLC tissues and cell lines and is associated with larger tumor size, lymphatic metastasis, later TNM stage, and shorter overall survival. It promotes NSCLC proliferation, migration, invasion, tumor growth and metastasis while inhibiting apoptosis. Mechanistically, circGLIS3 functions as a miRNA sponge and forms a positive feedback loop with miR-644a and PTBP1, in which circGLIS3 increases PTBP1 expression via miR-644a and PTBP1 enhances circGLIS3 circularization. |
| Confidence score: |
0.8684 |