| Expression pattern: |
DN |
| Associated gene: |
PTEN, p-AKT, PI3K/AKT signaling pathway |
| Associated microRNA: |
miR-942-5p |
| Biological function: |
Overexpression of hsa_circ_0001640 inhibited NSCLC cell proliferation, invasion and migration, promoted apoptosis, and suppressed xenograft tumor growth in vivo. |
| Molecular mechanism: |
hsa_circ_0001640 acts as a molecular sponge for miR-942-5p and inactivates the PI3K/AKT signaling pathway through the miR-942-5p/PTEN axis. |
| Biological pathway or process: |
PI3K/AKT (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Microarray; FISH / smFISH; Clinical Sample Validation; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High hsa_circ_0001640 expression was associated with smaller tumor size and longer survival in NSCLC patients; the study suggests it may be a biomarker and potential target for NSCLC detection and therapy. |
| Description: |
hsa_circ_0001640 is down-regulated in NSCLC tissues and cell lines and functions as a tumor suppressor. It sponges miR-942-5p to regulate PTEN and inhibit PI3K/AKT signaling, thereby suppressing proliferation, migration, invasion and tumor growth while promoting apoptosis. Higher expression is associated with smaller tumor size and longer survival, suggesting biomarker and therapeutic-target potential. |
| Confidence score: |
0.8635 |