| Expression pattern: |
UP |
| Associated gene: |
HIF-1alpha |
| Associated microRNA: |
miR-576-3p |
| Biological function: |
promotes proliferation, migration, invasion and glycolysis of gastric cancer cells; promotes tumor growth and metastasis in vivo |
| Molecular mechanism: |
ceRNA/miRNA sponge: circDNMT1 sponges miR-576-3p, relieving miR-576-3p-mediated repression of HIF-1alpha |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); glycolysis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Sanger Sequencing; FISH / smFISH; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Gel electrophoresis; Clinical Sample Validation; Survival Analysis; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Wound Healing Assay; Transwell Assay; Western Blot; In Vivo Animal Model |
| Clinical significance: |
High expression of circDNMT1 indicated poorer patient survival time; correlated with pathological T stage and pathological TNM stage |
| Description: |
circDNMT1 (Hsa_circ_0049224), derived from DNMT1, is up-regulated in gastric cancer tissues and cell lines and mainly localized in the cytoplasm. It promotes GC proliferation, migration, invasion and glycolysis by sponging miR-576-3p to derepress HIF-1alpha, enhancing tumor growth and metastasis in xenograft models, and high circDNMT1 is associated with poorer overall survival. |
| Confidence score: |
0.8521 |