circRNA basic information
circBase ID: -
Name: hsa_circ_SPECC1
Synonym: SPECC1 circular RNA / circSPECC1
Host Gene: SPECC1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005061
MONDO name: lung adenocarcinoma
Disease details: lung adenocarcinoma / LUAD
Disease DO ID:
 3910
Disease MeSH ID:
 C538231
Disease NCIt ID:
 C3512
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor samples; adjacent nontumor samples
Cell lines:

BEAS-2B; PC9; A549; H1975; H1299; H1650; H441
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: TUBB1, BCHE, BNIP3, LHFPL6, SLC39A4
Associated microRNA: miR-598-3p, miR-33a-3p, miR-497-5p, miR-369-3p
Biological function: promotes proliferation, migration, invasion and tumorigenesis/metastasis; promotes osimertinib (TKI) resistance by reducing apoptosis under osimertinib treatment
Molecular mechanism: ceRNA network: circSPECC1 sponges miRNAs (miR-598-3p, miR-33a-3p, miR-497-5p, miR-369-3p) affecting downstream mRNAs (TUBB1, BCHE, BNIP3, LHFPL6, SLC39A4)
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes); other pathway/process (other)
Detected method:
Q
S
Validation methods:

RNA-seq; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; EdU Staining; Wound Healing Assay; Transwell Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry
Clinical significance:

-
Description:

circSPECC1 is up-regulated in LUAD/NSCLC tumors and promotes malignant phenotypes (proliferation, migration, invasion, adhesion). circSPECC1 knockdown increases osimertinib-induced apoptosis and restores TKI sensitivity, and a ceRNA network suggests it sponges miR-598-3p/miR-33a-3p/miR-497-5p/miR-369-3p to affect downstream mRNAs (e.g., TUBB1, BCHE, BNIP3, LHFPL6, SLC39A4).
Confidence score:

0.6117
Other information
Title:

Circular RNA SPECC1 promoted tumorigenesis and osimertinib resistance in lung adenocarcinoma via a circular RNA-microRNA network.

Journal: Journal of thoracic disease
Published: 2024
PubMed ID: 39831223
Study type:

combined biological and clinical study
Data availability: http://www.ncbi.nlm.nih.gov/bioproject/707622
Code availability: -