| Expression pattern: |
UP |
| Associated gene: |
LASP1, Ago2 |
| Associated microRNA: |
miR-665 |
| Biological function: |
Exosomal circCOL1A2 promotes CRC cell proliferation, migration, invasion, EMT, tumor growth, and tumorigenesis. |
| Molecular mechanism: |
Exosomal circCOL1A2 acts as a ceRNA sponge for miR-665 to upregulate LASP1 expression, thereby modulating CRC malignant phenotypes. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circCOL1A2 expression was associated with poorer prognosis and lower overall survival rates in CRC patients; circCOL1A2 might be a valuable therapeutic target for CRC. |
| Description: |
circCOL1A2 is up-regulated in CRC tissues, CRC cells, and CRC cell-derived exosomes, and high expression is linked to poor prognosis. Exosomal circCOL1A2 promotes CRC proliferation, migration, invasion, EMT, and in vivo tumor growth by sponging miR-665 and increasing LASP1 expression. The study suggests circCOL1A2 as a potential therapeutic target in CRC. |
| Confidence score: |
0.8766 |