| Expression pattern: |
UP |
| Associated gene: |
TLR4, IL-6, IL-10, TNF-alpha, IL-1beta, IL-18, caspase 1, NLRP3 |
| Associated microRNA: |
miR-578 |
| Biological function: |
CircLARP1B inhibits proliferation and cell cycle progression, and promotes pyroptosis and inflammation in high glucose-induced renal mesangial cells. |
| Molecular mechanism: |
CircLARP1B acts as a sponge of miR-578 to regulate TLR4, thereby modulating proliferation, cell cycle, pyroptosis, and inflammation in HG-induced renal mesangial cells. |
| Biological pathway or process: |
proliferation (inhibits); cell cycle (inhibits); pyroptosis (promotes); inflammation (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; MTT; EdU Staining; Cell Cycle Assay; Flow Cytometry（Non-apoptosis/cycle）; ELISA; Western Blot; Luciferase Reporter Assay; Bioinformatics Analysis |
| Clinical significance: |
CircLARP1B may be a target for the treatment of DN. |
| Description: |
CircLARP1B is up-regulated in the blood of DN patients and in high glucose-treated human renal mesangial cells. It promotes pyroptosis and inflammatory factor release while inhibiting proliferation and cell cycle progression through the miR-578/TLR4 axis, suggesting therapeutic relevance in DN. |
| Confidence score: |
0.742 |