| Expression pattern: |
UP |
| Associated gene: |
NEK2 |
| Associated microRNA: |
miR-128-3p, hsa-miR-128-3p |
| Biological function: |
Promotes breast cancer cell proliferation, migration, invasion, metastasis and tumor growth; inhibits apoptosis and promotes cell cycle progression. |
| Molecular mechanism: |
circDOCK1 acts as a miR-128-3p sponge to derepress and upregulate NEK2, thereby promoting breast cancer progression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); cell cycle (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; MTT; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model |
| Clinical significance: |
circDOCK1/hsa-miR-128-3p/NEK2 axis may be a novel therapeutic target for breast cancer treatment; circDOCK1, miR-128-3p, and NEK2 levels correlate with breast cancer grades. |
| Description: |
circDOCK1 (hsa_circ_0007142) is up-regulated in breast cancer tissues and cell lines. The study reports that circDOCK1 promotes breast cancer progression by sponging miR-128-3p and derepressing NEK2, thereby enhancing proliferation, migration, invasion, metastasis and tumor growth while reducing apoptosis. The circDOCK1/hsa-miR-128-3p/NEK2 axis is proposed as a potential therapeutic target. |
| Confidence score: |
0.6542 |