| Expression pattern: |
UP |
| Associated gene: |
RAP1B |
| Associated microRNA: |
miR-140 |
| Biological function: |
Promotes paclitaxel resistance, proliferation, migration, invasion, EMT and tumor growth, and inhibits apoptosis in PTX-resistant ovarian cancer. |
| Molecular mechanism: |
circ_0000231 acts as a miR-140 sponge to positively regulate RAP1B and promote PTX-resistant ovarian cancer progression and chemoresistance. |
| Biological pathway or process: |
ceRNA regulation (promotes); chemoresistance (promotes); drug resistance (promotes); proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); apoptosis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circ_0000231 expression was significantly correlated with FIGO stage and may be a diagnostic and therapeutic target in ovarian cancer. |
| Description: |
circ_0000231 is up-regulated in paclitaxel-resistant ovarian cancer tissues and cells. It promotes PTX resistance, proliferation, migration, invasion and EMT while suppressing apoptosis, mainly through the circ_0000231/miR-140/RAP1B ceRNA axis. Knockdown of circ_0000231 increases PTX sensitivity in vitro and in a cell line-derived xenograft model. |
| Confidence score: |
0.817 |