circRNA basic information
circBase ID: -
Name: hsa_circ_CCDC66
Synonym: -
Host Gene: CCDC66
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: membrane
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005061
MONDO name: lung adenocarcinoma
Disease details: lung adenocarcinoma
Disease DO ID:
 3910
Disease MeSH ID:
 C538231
Disease NCIt ID:
 C3512
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissue; non-tumor lung tissue (NTLT)
Cell lines:

H1975; H23; A549; H2009
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: FAK, c-Met, HGF, nAchRalpha7, EGFR
Associated microRNA: -
Biological function: Promotes EMT and invasion/metastatic potential; increases drug resistance in lung adenocarcinoma cells.
Molecular mechanism: HGF/c-Met and FAK signaling regulate cirRNA CCDC66 expression; cirRNA CCDC66 is suggested to adsorb miRNA and may act on the ER where miRNA maturation/adsorption occurs, contributing to EMT marker expression and invasion.
Biological pathway or process:

EMT (promotes); invasion (promotes); metastasis (promotes); drug resistance (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Transfection; Transwell Assay; Bioinformatics Analysis
Clinical significance:

-
Description:

cirRNA CCDC66 is up-regulated in lung adenocarcinoma specimens/cells and is regulated by HGF/c-Met and FAK (and negatively by nAchRalpha7). Functional perturbation shows it promotes EMT-marker expression and invasion, and is implicated in drug-resistance phenotypes in LADC cells.
Confidence score:

0.3963
Other information
Title:

The role of HGF-MET pathway and CCDC66 cirRNA expression in EGFR resistance and epithelial-to-mesenchymal transition of lung adenocarcinoma cells.

Journal: Journal of hematology & oncology
Published: 2018
PubMed ID: 29855336
Study type:

combined biological and clinical study
Data availability: Supplementary material (10.1186/s13045-018-0557-9)
Code availability: -