circRNA basic information
circBase ID: hsa_circ_0026782
Name: hsa_circ_ITGA7
Synonym: circITGA7
Host Gene: ITGA7
Genomic location(hg19): chr12:56094682-56094938:-
Genomic location(hg38): chr12:55700898-55701154:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

colorectal cancer samples; paired adjacent noncancerous colorectal tissues; CRC tissues; adjacent noncancerous tissues; orthotopic tumours; subcutaneous xenografts; liver metastases
Cell lines:

SW480; RKO; Caco-2; SW620; LoVo; HCT116; DLD1; FHC; 293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: NF1, Ras, ERK, AKT, ITGA7, RREB1
Associated microRNA: miR-370-3p
Biological function: circITGA7 suppresses CRC cell proliferation, migration, invasion, tumour growth and metastasis in vitro and in vivo; knockdown promotes CRC cell proliferation, migration and xenograft growth.
Molecular mechanism: circITGA7 acts as a cytoplasmic miRNA sponge for miR-370-3p, upregulates NF1, suppresses Ras pathway activation and downstream ERK/AKT phosphorylation, and upregulates host gene ITGA7 transcription by suppressing RREB1 via the Ras pathway.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); cell cycle (inhibits); PI3K/AKT (inhibits); ERK (inhibits); ceRNA regulation (other); other pathway/process (inhibits)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; ChIP / ChIP-seq; Transfection; MTT; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; ROC Analysis; Bioinformatics Analysis
Clinical significance:

circITGA7 has potential as a biomarker for CRC diagnosis; circITGA7 expression levels were inversely correlated with tumour size, lymph metastasis, distant metastasis and TNM stage.
Description:

circITGA7 is a human ITGA7-derived circRNA that is significantly downregulated in colorectal cancer tissues and cell lines and is associated with CRC progression and diagnostic potential. Functionally, circITGA7 acts as a tumour suppressor, inhibiting CRC proliferation, migration, invasion, tumour growth and metastasis. Mechanistically, cytoplasmic circITGA7 binds miR-370-3p to upregulate NF1 and suppress Ras signaling, and also promotes ITGA7 transcription by suppressing RREB1 via the Ras pathway.
Confidence score:

0.8837
Other information
Title:

Circular RNA circITGA7 inhibits colorectal cancer growth and metastasis by modulating the Ras pathway and upregulating transcription of its host gene ITGA7.

Journal: The Journal of pathology
Published: 2018
PubMed ID: 29943828
Study type:

combined biological and clinical study
Data availability: -
Code availability: -