circRNA basic information
circBase ID: -
Name: hsa_circ_FMN2
Synonym: Circ_0005100 / circ_FMN2
Host Gene: FMN2
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
5672, 9256
Disease MeSH ID:
-
Disease NCIt ID:
C4978
Disease ICD11 ID:
-
Disease OMIM ID:
114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

serum; serum exosomes; tumor tissues

Cell lines:

HCT116; KM12; NCM460

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: MSI1, Ago2
Associated microRNA: miR-338-3p
Biological function: Promotes CRC cell proliferation, cell cycle progression, colony formation, viability, migration, invasion, EMT and tumor growth, and suppresses apoptosis.
Molecular mechanism: Exosomal circ_FMN2 functions as a miR-338-3p sponge to upregulate MSI1, thereby promoting CRC progression.
Biological pathway or process:

proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (other)

Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis

Clinical significance:

Exosomal circ_FMN2 might be a target for CRC treatment.

Description:

Serum exosomal circ_FMN2 is upregulated in CRC patients and CRC cells. It promotes CRC proliferation, metastasis, EMT and tumor growth while inhibiting apoptosis, mainly by sponging miR-338-3p and upregulating MSI1. The study suggests exosomal circ_FMN2 as a potential therapeutic target for CRC.

Confidence score:

0.8006

Other information
Title:

Exosomal Circ_FMN2 Derived from the Serum of Colorectal Cancer Patients Promotes Cancer Progression by miR-338-3p/MSI1 Axis.

Journal: Applied biochemistry and biotechnology
Published: 2023
PubMed ID: 36995659
Study type:

combined biological and clinical study

Data availability: The data that support the findings of this study are available from the corresponding author upon reasonable request.
Code availability: -